US 11,858,979 B2
Fibronectin based scaffold domain proteins that bind PCSK9
Ray Camphausen, Wayland, MA (US); Jonathan H. Davis, Auburndale, MA (US); Sharon T. Cload, Cambridge, MA (US); Fabienne M. Denhez, Arlington, MA (US); Amna Saeed-Kothe, West Roxbury, MA (US); Dasa Lipovsek, Cambridge, MA (US); Ching-Hsiung Frederick Lo, Pennington, NJ (US); Chee Meng Low, Allston, MA (US); Bowman Miao, Churchville, PA (US); Tracy S. Mitchell, Andover, MA (US); Rex A. Parker, Titusville, NJ (US); Ginger C. Rakestraw, Cambridge, MA (US); Katie A. Russo, Watertown, MA (US); and Doree F. Sitkoff, Dresher, PA (US)
Assigned to Bristol-Meyers Squibb Company, Princeton, NJ (US)
Filed by Bristol-Myers Squibb Company, Princeton, NJ (US)
Filed on Feb. 16, 2021, as Appl. No. 17/177,179.
Application 17/177,179 is a continuation of application No. 15/819,925, filed on Nov. 21, 2017, granted, now 10,947,297.
Application 15/819,925 is a continuation of application No. 14/956,698, filed on Dec. 2, 2015, granted, now 9,856,309, issued on Jan. 2, 2018.
Application 14/956,698 is a continuation of application No. 13/835,639, filed on Mar. 15, 2013, granted, now 9,234,027, issued on Jan. 12, 2016.
Application 13/835,639 is a continuation of application No. 13/085,864, filed on Apr. 13, 2011, granted, now 8,420,098, issued on Apr. 16, 2013.
Claims priority of provisional application 61/330,731, filed on May 3, 2010.
Claims priority of provisional application 61/323,562, filed on Apr. 13, 2010.
Prior Publication US 2021/0277089 A1, Sep. 9, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/02 (2006.01); C12N 1/20 (2006.01); C12N 15/00 (2006.01); C07H 21/04 (2006.01); C07K 14/78 (2006.01); A61K 38/39 (2006.01); C12N 9/64 (2006.01); A61K 47/60 (2017.01); A61K 47/54 (2017.01); C07K 16/18 (2006.01); C07K 16/40 (2006.01)
CPC C07K 14/78 (2013.01) [A61K 38/39 (2013.01); A61K 47/549 (2017.08); A61K 47/60 (2017.08); C07K 16/18 (2013.01); C07K 16/40 (2013.01); C12N 9/6424 (2013.01); C07K 2318/20 (2013.01); C07K 2319/30 (2013.01); C07K 2319/31 (2013.01); C07K 2319/70 (2013.01)] 10 Claims
 
1. An isolated nucleic acid molecule encoding a polypeptide comprising a fibronectin type III tenth (10Fn3) domain wherein the 10Fn3 has a BC, DE and FG loop, and wherein the BC loop comprises SEQ ID NO:14, the DE comprises SEQ ID NO:25 and the FG loop comprises SEQ ID NO:37, and wherein the polypeptide binds PCSK9.