US 11,858,973 B2
Granulysin, method of obtaining same, and uses
Luis Alberto Anel Bernal, Saragossa (ES); Raquel Ibañez Perez, Saragossa (ES); Patricia Guerrero Ochoa, Saragossa (ES); Luis Martinez Lostao, Saragossa (ES); Blanca Conde Guerri, Saragossa (ES); Ramón Hurtado Guerrero, Saragossa (ES); Ana Laura Sanz Alcober, Majadahonda (ES); and Rocio Navarro Ortiz, Majadahonda (ES)
Assigned to UNIVERSIDAD DE ZARAGOZA, Saragossa (ES); FUNDACIÓN AGENCIA ARAGONESA PARA LA INVESTIGACIÓN Y EL DESARROLLO (ARAID), Saragossa (ES); and FUNDACIÓN PARA LA INVESTIGACIÓN BIOMÉDICA HOSPITAL UNIVERSITARIO PUERTA DE HIERRO MAJADAHONDA, Majadahonda (ES)
Appl. No. 17/263,122
Filed by UNIVERSIDAD DE ZARAGOZA, Saragossa (ES); FUNDACIÓN AGENCIA ARAGONESA PARA LA INVESTIGACIÓN Y EL DESARROLLO (ARAID), Saragossa (ES); and FUNDACIÓN PARA LA INVESTIGACIÓN BIOMÉDICA HOSPITAL UNIVERSITARIO PUERTA DE HIERRO MAJADAHONDA, Majadahonda (ES)
PCT Filed Jul. 24, 2019, PCT No. PCT/EP2019/069979
§ 371(c)(1), (2) Date Jan. 25, 2021,
PCT Pub. No. WO2020/020978, PCT Pub. Date Jan. 30, 2020.
Claims priority of application No. ES201830768 (ES), filed on Jul. 26, 2018.
Prior Publication US 2021/0340196 A1, Nov. 4, 2021
Int. Cl. C07K 14/47 (2006.01); A61K 38/00 (2006.01); A61P 35/00 (2006.01); C07K 16/30 (2006.01); C12N 15/81 (2006.01); C12R 1/84 (2006.01); A61K 39/00 (2006.01)
CPC C07K 14/47 (2013.01) [A61P 35/00 (2018.01); C07K 16/3007 (2013.01); C12N 15/815 (2013.01); A61K 38/00 (2013.01); A61K 2039/505 (2013.01); C07K 2317/622 (2013.01); C07K 2319/02 (2013.01); C12R 2001/84 (2021.05)] 21 Claims
 
1. A pharmaceutical composition comprising a molecule comprising:
a) a recombinant antibody targeting a tumor antigen, wherein the recombinant antibody comprises anti-CEA scFv MFE23 of SEQ ID No: 2; and
b) a granulysin polypeptide comprising SEQ ID NO:1,
wherein said recombinant antibody and said granulysin polypeptide are part of a single polypeptide chain; and
wherein the recombinant antibody is located at an N-terminal end of the single polypeptide chain and is physically bound by a peptide connector to the granulysin polypeptide.