US 11,858,961 B2
Solutions of quaternary and tertiary ammonium cation detergents to denature proteins, and kits for making them
Michael J. Kimzey, Santa Clara, CA (US); Francis T. Haxo, Santa Clara, CA (US); and Vaishali Sharma, Santa Clara, CA (US)
Assigned to Agilent Technologies, Inc., Santa Clara, CA (US)
Filed by Agilent Technologies, Inc., Santa Clara, CA (US)
Filed on Aug. 10, 2021, as Appl. No. 17/398,763.
Application 17/398,763 is a continuation of application No. 16/323,247, granted, now 11,111,268, previously published as PCT/US2017/047455, filed on Aug. 17, 2017.
Claims priority of provisional application 62/376,342, filed on Aug. 17, 2016.
Prior Publication US 2021/0371454 A1, Dec. 2, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 1/00 (2006.01); C07K 1/113 (2006.01); C11D 1/62 (2006.01); C11D 17/00 (2006.01)
CPC C07K 1/1136 (2013.01) [C07K 1/113 (2013.01); C11D 1/62 (2013.01); C11D 17/00 (2013.01)] 20 Claims
 
1. A solution for denaturing a glycoprotein or glycopeptide of interest in vitro, said solution comprising (A) said glycoprotein or glycopeptide of interest and (B) an effective amount of an ammonium cation sulfate or sulfonate detergent comprising (a) an aliphatic chain of 8-24 carbons, (b) a sulfate or sulfonate anion and (c) a tertiary or quaternary ammonium cation, wherein said aliphatic chain is covalently attached to said anion.