US 11,857,957 B2
Fluidic devices with reaction wells and uses thereof
Deepak Solomon, Pleasanton, CA (US); and Connor Sullivan, Pleasanton, CA (US)
Assigned to Unchained Labs, Pleasanton, CA (US)
Filed by Unchained Labs, Pleasanton, CA (US)
Filed on Oct. 25, 2019, as Appl. No. 16/664,106.
Application 16/664,106 is a continuation of application No. PCT/US2018/029692, filed on Apr. 27, 2018.
Claims priority of provisional application 62/491,961, filed on Apr. 28, 2017.
Prior Publication US 2020/0055051 A1, Feb. 20, 2020
Int. Cl. B01L 3/00 (2006.01)
CPC B01L 3/502738 (2013.01) [B01L 2200/0642 (2013.01); B01L 2300/087 (2013.01); B01L 2400/0688 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A fluidic device comprising:
a first fluid transport channel;
a reaction well;
an overflow channel;
a fluidic connection;
an air control valve;
a port for the air control valve; and
a second fluid transport channel,
wherein:
the first fluid transport channel is in direct fluidic communication with the overflow channel and the reaction well;
the overflow channel is further in direct fluidic communication with the second fluid transport channel and the fluidic connection; and
the fluidic connection is further in direct fluidic communication with the reaction well and the air control valve.
 
4. A fluidic device comprising:
an air control valve;
a fluidic connection;
a reaction well;
air valve constriction channel;
an overflow channel;
a first fluid transport channel; and
a second fluid transport channel,
wherein:
the first fluid transport channel is in direct fluidic communication with the overflow channel and the reaction well;
the overflow channel is further in direct fluidic communication with the reaction well, the air valve constriction channel, and the second fluid transport channel;
the reaction well is further in direct fluidic communication with the fluidic connection;
the fluidic connection is further in direct fluidic communication with the air valve constriction channel and the air control valve; and
the second fluid transport channel is further in direct fluidic communication with the air valve constriction channel.
 
7. A fluidic system comprising:
a) a first fluid transport channel;
b) a second fluid transport channel;
c) a plurality of fluidic devices arranged in series, each fluidic device being positioned between the first fluid transport channel and the second fluid transport channel, wherein each fluidic device comprises:
a first intradevice fluid transport channel;
a reaction well;
an overflow channel;
a fluidic connection;
an air control valve;
a air valve constriction channel;
a port for the air control valve; and
a second intradevice fluid transport channel; and
d) a sample port and sample channel for at least one of the plurality of fluidic devices; wherein:
the first fluid transport channel is in direct fluidic communication with the sample channel and the first intradevice fluid transport channel of the first fluidic device;
the first intradevice fluid transport channel of each fluidic device is in direct fluidic communication with the reaction well and the overflow channel thereof;
the fluidic connection of each fluidic device is in direct fluidic communication with the reaction well, the air control valve, and the air valve constriction channel thereof;
the second intradevice fluid transport channel of each fluidic device is in direct fluidic communication with the overflow channel and the air valve constriction channel thereof;
the second intradevice fluid transport channel of each fluidic device is continuous with the first intradevice fluid transport channel of a subsequent fluidic device in the plurality of fluidic devices adjoined to one another and the first intradevice fluid transport channel and the second intradevice fluid transport channel are in direct fluidic communication with the sample channel; and
the second intradevice fluid transport channel of the last fluidic device in the series is continuous with the second fluid transport channel.