CPC A61K 49/223 (2013.01) [A61B 8/481 (2013.01); A61K 49/226 (2013.01); A61K 49/227 (2013.01); C07F 9/09 (2013.01); C07F 9/106 (2013.01)] | 21 Claims |
1. A method for preparing a phospholipid suspension useful for preparing lipid-encapsulated gas microspheres comprising
forming a phospholipid solution that comprises (a) a DPPA, DPPC, and MPEG5000-DPPE phospholipid blend and propylene glycol, and (b) calcium and/or magnesium at a combined concentration of less than 0.7 ppm, wherein the phospholipid blend is formed in the presence of one or two but not all three of methanol, toluene and methyl t-butyl ether (MTBE), and
combining the phospholipid solution with an aqueous solution to form a phospholipid suspension.
|