US 11,857,616 B2
Recombinant measles virus expressing zika virus prM and E proteins
Frederic Tangy, Les Lilas (FR); and Etienne Simon-Loriere, Paris (FR)
Assigned to INSTITUT PASTEUR, Paris (FR); and CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR)
Appl. No. 16/618,823
Filed by INSTITUT PASTEUR, Paris (FR); and CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR)
PCT Filed Jun. 6, 2018, PCT No. PCT/EP2018/064943
§ 371(c)(1), (2) Date Dec. 3, 2019,
PCT Pub. No. WO2018/224573, PCT Pub. Date Dec. 13, 2018.
Claims priority of application No. 17305676 (EP), filed on Jun. 7, 2017.
Prior Publication US 2020/0237893 A1, Jul. 30, 2020
Int. Cl. A61K 39/12 (2006.01); C07K 14/005 (2006.01); C12N 7/00 (2006.01); A61K 39/00 (2006.01)
CPC A61K 39/12 (2013.01) [C07K 14/005 (2013.01); C12N 7/00 (2013.01); A61K 2039/5254 (2013.01); A61K 2039/53 (2013.01); C12N 2760/18421 (2013.01); C12N 2760/18423 (2013.01); C12N 2770/24122 (2013.01); C12N 2770/24123 (2013.01); C12N 2770/24134 (2013.01)] 24 Claims
 
1. A nucleic acid construct which comprises:
(1) a polynucleotide encoding at least (i) the precursor of membrane (prM) protein of a Zika virus (ZIKV), and the envelope (E) protein of a ZIKV or a truncated version thereof of SEQ ID NO: 26, SEQ ID NO: 29 and SEQ ID NO: 32, or (ii) the E protein of a ZIKV or the truncated version thereof of SEQ ID NO: 26, SEQ ID NO: 29 and SEQ ID NO: 32; and
(2) a cDNA molecule encoding a full-length, infectious antigenomic (+) RNA strand of a live-attenuated measles virus (MV) vaccine strain;
wherein the polynucleotide encoding at least (i) the prM protein of the ZIKV, and the E protein of the ZIKV or the truncated version thereof, or (ii) the E protein of the ZIKV or the truncated version thereof and the cDNA molecule are operatively linked;
the nucleic acid construct comprising the following polynucleotides from 5′ to 3′:
(a) a polynucleotide encoding the N protein of the MV;
(b) a polynucleotide encoding the P protein of the MV;
(c) the polynucleotide encoding at least (i) the prM protein of the ZIKV, and the E protein of the ZIKV or the truncated version thereof, or (ii) the E protein of the ZIKV or the truncated version thereof;
(d) a polynucleotide encoding the M protein of the MV;
(e) a polynucleotide encoding the F protein of the MV;
(f) a polynucleotide encoding the H protein of the MV; and
(g) a polynucleotide encoding the L protein of the MV;
wherein said polynucleotides are operably linked in the nucleic acid construct and under a control of viral replication and transcription regulatory sequences.