CPC A01N 37/06 (2013.01) [A23D 9/013 (2013.01); A23D 9/02 (2013.01); A23K 20/158 (2016.05); A23L 33/115 (2016.08); A61K 8/361 (2013.01); A61K 8/9706 (2017.08); A61K 8/9728 (2017.08); A61K 8/99 (2013.01); A61K 31/202 (2013.01); A61K 36/06 (2013.01); A61Q 19/00 (2013.01); C11B 1/025 (2013.01); C11B 3/10 (2013.01); C11B 3/12 (2013.01); C11C 1/005 (2013.01); C11C 3/10 (2013.01); C12N 1/28 (2013.01); C12P 7/6427 (2013.01); C12P 7/6436 (2013.01); C12P 7/6463 (2013.01)] | 20 Claims |
1. A microbial oil comprising:
arachidonic acid (ARA) or Mead acid in a fatty acid alkyl ester form or in a free fatty acid form at a content of at least 50% by weight of the total weight of fatty acids in the microbial oil; and
one or more trans isomers of ARA or Mead acid at a total content of 0.0001% to 3.0% by weight of the total weight of fatty acids in the microbial oil,
wherein the microbial oil comprises a mixture of fatty acid alkyl esters or free fatty acids obtained by the steps of i) performing hydrolysis or alkyl esterification of a crude oil obtained from a microbial biomass from a microorganism capable of producing ARA or Mead acid to produce a hydrolyzed or esterified crude oil; and ii) purifying the hydrolyzed or esterified crude oil by rectification using a distillation column containing a structured packing, under conditions including a column bottom temperature of 160° C. to 230° C. and a minimum pressure in a distillation column of 0.1 Pa to 30 Pa,
wherein the one or more trans isomers of ARA or Mead acid are generated during the rectification step as thermally-produced fatty acids.
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