US 11,856,945 B2
Methods and systems for preparing and preserving a biological sample
Srikanth Singamaneni, St. Louis, MO (US); Congzhou Wang, St. Louis, MO (US); Jeremiah J. Morrissey, St. Louis, MO (US); and Evan D. Kharasch, St. Louis, MO (US)
Assigned to Washington University, St. Louis, MO (US)
Appl. No. 16/606,482
Filed by Srikanth Singamaneni, St. Louis, MO (US); Congzhou Wang, St. Louis, MO (US); Jeremiah J. Morrissey, St. Louis, MO (US); and Evan D. Kharasch, St. Louis, MO (US)
PCT Filed Apr. 20, 2018, PCT No. PCT/US2018/028591
§ 371(c)(1), (2) Date Oct. 18, 2019,
PCT Pub. No. WO2018/195438, PCT Pub. Date Oct. 25, 2018.
Claims priority of provisional application 62/488,231, filed on Apr. 21, 2017.
Prior Publication US 2020/0305416 A1, Oct. 1, 2020
Int. Cl. G01N 33/68 (2006.01); G01N 21/33 (2006.01); G01N 21/64 (2006.01); G01N 33/52 (2006.01); C07C 309/05 (2006.01); C07C 309/73 (2006.01); A01N 1/02 (2006.01); G01N 1/40 (2006.01)
CPC A01N 1/0231 (2013.01) [G01N 1/4044 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method of preparing a biological sample comprising:
contacting a preserving agent with a biological sample to form a mixture, wherein the preserving agent is selected from at least one of a metal-organic framework (MOF) encapsulant or a precursor forming a MOF encapsulant, and wherein the biological sample comprises a biofluid comprising at least one target analyte; and
drop casting the mixture onto a fibrous substrate;
wherein the fibrous substrate is made of paper or cellulose; and
wherein a crystal interface is created between the protein and the fibrous substrate.